![]() ![]() Sequence reads were trimmed using CLC Genomics Workbench - Quality limit: 0.05. TruSeq stranded mRNA Kit (Illumina, San Diego, USA) with TruSeq RNA Adapter Box Set A and B (Illumina, San Diego, USA) and protocol 1000000040498v00 (Elution-2-Frag-Prime step: 4 minutes at 94☌ 15 cycles enrichment) ![]() Total RNA was extracted using a NucleoSpin RNA kit (Macherey-Nagel). Plants were shifted to the high-light chambers for an additional growth- and acclimatization period of one week under constant light with 60-70 µmol m-2 s-1 (growth light GL) at 20-22☌. Marchantia polymorpha lines BoGa, Mpb-gata1-1, Mpb-gata1-2, and MpB-GATA1ox3 were grown on 1/2 Gamborg's B5 medium in long day conditions (16L/8D) at 60 µmol m-2 s-1 and 22☌ for two weeks. One third of the plants were harvested and immediately frozen in liquid nitrogen each before the shift to high-light (t0), after six hours of high-light (t6HL) or six hours of mock (t6G¬L). The light treatments consisted of either six hours of high-light (HL) with 1200 µmol m-2 s-1 at 20-22☌ (t6HL) or a six hour mock treatment under constant light with 60-70 µmol m-2 s-1 at 20-22☌ (t6GL). GEO help: Mouse over screen elements for information. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |